p53 anticorps (Alexa Fluor 647)

N° du produit ABIN2689872

L’anticorps Souris Monoclonal anti-p53 a été validé pour ICS. Il convient pour détecter p53 dans des échantillons de Humain. Il y a 8+ publications disponibles.

Aperçu rapide pour p53 anticorps (Alexa Fluor 647) (ABIN2689872)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: L82

Détail du produit anti-p53 anticorps (Alexa Fluor 647)

Marque: BD Phosflow™

Attributs du produit: The p53 protein is critical to regulation of normal cell growth and proliferation and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. Post-translational acetylation of p53 enhances its DNA-binding activity. There are multiple factors that affect p53 acetylation, thereby modulating cellular proliferation and apoptosis. The L82-51 monoclonal antibody recognizes acetylated lysine 382 (acK382) in the C-terminal region of p53. Analysis of p53 (acK382) in lymphocytes. Human peripheral blood mononuclear cells (PBMC) were either treated with 0.4 μM Trichostatin A (Sigma, Cat. No. T8552) plus 0.4 μM Adriamycin (Doxorubicin hydrochloride, Sigma, Cat. No. D1515) for 24 hours (shaded histogram) or untreated (open histogram). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-p53 (acK382). Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray™ bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control (left blot) and Trichostatin A-plus-Adriamycin-treated (right blot) PBMC. p53 (acK382) is identified as a band of 53 kDa in the treated cells.

BD Phosflow™ Alexa Fluor® 647 Mouse anti-p53 (acK382) - Alexa Fluor® 647 - Clone L82-51 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human p53 acetylated Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-p53 (Alexa Fluor 647) (ABIN2689872)

Nishino, Wang, Mochizuki-Kashio, Osawa, Nakauchi, Iwama: "Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase." dans: PLoS ONE, Vol. 6, Issue 9, pp. e24298, (2011) (PubMed).

Soliman, Riabowol: "After a decade of study-ING, a PHD for a versatile family of proteins." dans: Trends in biochemical sciences, Vol. 32, Issue 11, pp. 509-19, (2007) (PubMed).

Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." dans: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, (2005) (PubMed).

Pedeux, Sengupta, Shen, Demidov, Saito, Onogi, Kumamoto, Wincovitch, Garfield, McMenamin, Nagashima, Grossman, Appella, Harris: "ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation." dans: Molecular and cellular biology, Vol. 25, Issue 15, pp. 6639-48, (2005) (PubMed).

Hofmann, Möller, Sirma, Zentgraf, Taya, Dröge, Will, Schmitz: "Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2." dans: Nature cell biology, Vol. 4, Issue 1, pp. 1-10, (2002) (PubMed).

Saito, Goodarzi, Higashimoto, Noda, Lees-Miller, Appella, Anderson: "ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation." dans: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 12491-4, (2002) (PubMed).

Ito, Lai, Zhao, Saito, Hamilton, Appella, Yao: "p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2." dans: The EMBO journal, Vol. 20, Issue 6, pp. 1331-40, (2001) (PubMed).

Vaziri, Dessain, Ng Eaton, Imai, Frye, Pandita, Guarente, Weinberg: "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase." dans: Cell, Vol. 107, Issue 2, pp. 149-59, (2001) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: Synonyms: TP53 (acK382)

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg